Poop-encrusted chamber pots from the Roman Empire reveal oldest known human cases of Crypto parasite

Urine and fecal residue encrusted on the inside of ancient Roman chamber pots unearthed in Bulgaria has revealed the world's oldest known evidence of humans infected with the Cryptosporidium parasite, which causes acute gastrointestinal distress.In the first century, the Romans established a province called Moesia Inferior in the Balkan Peninsula, which includes the modern country of Bulgaria. Roman legions were tasked with defending the imperial border from the Goths, primarily from a fortress called Novae (near present-day Svishtov) and a town known as Marcianopolis (modern-day Devnya). While excavating at Novae and Marcianopolis in Bulgaria, archaeologists recovered four chamber pots, whose long-dried contents have revealed new information about health and disease in the Roman Empire.Using ELISA (short for "enzyme-linked immunosorbent assay") ‪—‬ a laboratory test that can detect bacteria, parasites and viruses in a sample of bodily fluid ‪—‬ the researchers identified three pathogens in the chamber pot samples: the protozoan Entamoeba histolytica, the parasite Cryptosporidium parvum, and the tapeworm Taenia. All three pathogens infect the human gut and can cause gastrointestinal distress, including diarrhea and stomach pain.Previous ancient-parasite studies have shown that Roman soldiers on the frontiers of the empire dealt with intestinal worms and the parasite Giardia, as did